Video: A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53
jove.com
Video: A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
19.2K Views. University of Cambridge. The overall goal of this protocol, is to clone multiple guide RNAs into one CRISPR-concatemer vector and to achieve highly efficient electroporation in mouse intestinal organoids, in order to obtain simultaneous knockout of multiple genes. This method can greatly improve the efficiency of lost of function ...
18.5K viewsJul 12, 2017
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