Inducible protein overexpression in bacteria with the T7 expression system
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YouTubethe bumbling biochemist
Inducible protein overexpression in bacteria with the T7 expression system
Want a lot of protein made on demand? And you don’t just want it expressed - you want it over-expressed? Take a page out of the book of T7-phage! In recombinant protein over-expression in bacteria, I can stick the genetic instructions for a protein I’m interested in into a plasmid vector (like pET28) and get the bacteria to devote almost ...
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